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重组金黄色葡萄球菌蛋白A(蛋白A)
重组金黄色葡萄球菌蛋白A(蛋白A)
英文名称:Recombinant Staphylococcal Protein A SPA总访问:18940
国产/进口:进口半年访问:2816
产地/品牌:进口原装产品类别:生化试剂
规       格:PRO-356 最后更新:2019-11-12
货       号:
CAS   号:
参考报价:10mg/480元,10x10mg/4200元,1gr/32400元
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Certificate of Analysis and Data Sheet


Source:
E.Coli
Catalog No.
PRO-356

Background:
Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).


Description :
Recombinant Staphylococal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain containing 178 amino acids and having a molecular mass of 23,307 Dalton.
Recombinant Staphylococal Protein A is purified by proprietary chromatographic techniques.


Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation:
Recombinant Protein A was lyophilized from a concentrated (1mg/ml) solution containing no additives.


Stability:

Recombinant Protein A although stable at 4 C for 21 days, should be stored desiccated below -20 C for long term periods .
Please avoid freeze-thaw cycles.


Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.


Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.


Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of Recombinant Protein A.


Activity:
Greater than 95% binding activity to human IgG.


Usage:
Prospec''s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


Gene:
Name:spa
OrderedLocusNames:GN:SAOUHSC_00069#ORF">SAOUHSC_00069


Protein synonyms/aliases:
Immunoglobulin G-binding protein A precursor (IgG-binding protein A)
(Staphylococcal protein A).

 

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